Evaluation of Changes in NFKB Gene Expression Following Epstein-Barr Virus and its Participation in the Half-Life of Patients With Acute Epstein-Barr Positive Lymphoblastic Leukemia

Aim: Leukemia targets are one of the most common childhood malignancies. Epstein-Barr virus is a tumorigenic virus of the herpes family of viruses and causes primary infection in young children. The aim of this study was to evaluate the increase in NFKB expression following EBV virus and its contribution to the half-life of EBV-positive acute lymphoblastic leukemia patients. Materials and Methods: In this case-control study, blood samples of 60 patients referred to Tabriz Children's Hospital during 6 months in 1398 were examined. RNA extraction was performed from the collected samples and the quantity and quality of the extracted RNA were controlled by nanodrop device and gel electrophoresis. CDNA synthesis was performed from RNA extracted from the sample. NFKB gene expression was assessed using Real Time PCR. Data were analyzed using T-test. Finding: The results showed that EBV virus decreased the expression of NFKB gene in patients with acute lymphoblastic leukemia and also the expression of NFKB gene in patients with acute lymphoblastic leukemia increased significantly compared to the control group p <0.05.  Conclusion: The NFKB gene can be used as a precursor to the diagnosis of acute lymphoblastic leukemia, which requires further studies in this field. It also requires further studies on EBV infection and acute lymphoblastic disease.